葛根素预处理对大鼠缺血再灌注心肌细胞凋亡的影响

被引：9

作者：

吴青

周祖玉

陶大昌

机构：

[1] 四川省卫生干部管理学院生理教研室

[2] 四川大学基础与法医学院生理教研室

[3] 四川大学华西医学院遗传研究室

来源：

基础医学与临床 | 2005年 / 02期

关键词：

葛根素; 心肌细胞; 缺血/再灌注; 凋亡;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

摘要：

目的观察葛根素(Pur)预处理对缺血再灌注(I/R)心肌细胞凋亡的干预作用,并探讨其作用的可能机理。方法将原代培养的新生大鼠心肌细胞随机分为(1)正常对照组及正常+Pur组;(2 )模拟缺血组及缺血+Pur组;(3)模拟再灌注组及再灌注+Pur组。末端标记法(TUNEL法)检测心肌细胞凋亡,流式细胞仪检测细胞凋亡百分率,透射电镜观察心肌细胞凋亡的形态学特征、免疫组织化学法检测Bcl 2 /Bax蛋白表达,并检测胞内Ca2 + 浓度。结果(1)心肌细胞I/R后电镜观察到典型的凋亡超微结构特征;TUNEL染色可见心肌细胞凋亡阳性反应;免疫组化检测发现Bcl 2蛋白表达下调,Bax蛋白表达上调;胞内明显Ca2 + 超载。(2 )葛根素可显著降低I/R心肌细胞内Ca2 + 浓度、降低凋亡指数和凋亡百分率、上调Bcl 2蛋白表达、下调Bax蛋白表达。结论葛根素具有抗心肌细胞凋亡的作用,其机理可能与抑制胞内Ca2 + 超载,干预Bcl 2 /Bax蛋白表达有关。

引用

页码：147 / 151

页数：5

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