EGCG改善D-gal诱导阿尔茨海默病模型大鼠认知障碍机制研究

被引：5

作者：

姚维范

刘明妍

钟欣

王崴

杜可

杨时伦

毛瑞琨

魏敏杰

机构：

[1] 中国医科大学药学院药理学教研室

来源：

临床军医杂志 | 2016年 / 44卷 / 06期

关键词：

EGCG; p75NTR通路; 凋亡; 认知障碍; D-gal诱导AD模型大鼠;

D O I：

10.16680/j.1671-3826.2016.06.07

中图分类号：

R749.16 [];

学科分类号：

摘要：

目的探讨表没食子儿茶素没食子酸酯(EGCG)通过抑制p75NTR介导的凋亡通路相关因子改善D-gal诱导阿尔茨海默病(AD)模型大鼠学习记忆障碍的作用及其机制。方法以Morris水迷宫观察动物行为学变化,TUNEL法检测神经细胞凋亡情况,同时采用免疫组化及Western blot法检测其脑内Cleaved-Caspase-3表达水平,并用Western blot法检测p75NTR介导的凋亡通路相关蛋白表达水平。结果 EGCG明显改善D-gal诱导AD模型大鼠学习记忆障碍,抑制其脑内TUNEL阳性细胞表达,降低凋亡特征性蛋白Cleaved-Caspase-3的表达水平。同时,EGCG可通过抑制p75NTR介导的凋亡相关通路,显著降低p75ICD表达水平及其下游JNK2、c-Jun磷酸化水平,降低下游凋亡相关蛋白p53的表达水平,从而发挥抗凋亡、改善学习记忆障碍的抗AD作用。结论 EGCG可能通过抑制p75NTR介导的JNK/c-Jun/p53凋亡相关信号通路,抑制D-gal诱导AD模型大鼠脑内神经细胞凋亡,从而改善其认知障碍。

引用

页码：574 / 578

页数：5

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