A型产气荚膜梭菌α-毒素基因的克隆与核苷酸序列分析

被引:3
作者
赵志军
许崇波
曾瑾
邓光存
王玉炯
机构
[1] 宁夏大学生命科学学院
[2] 宁夏大学生命科学学院 宁夏银川
[3] 宁夏银川
关键词
A型产气荚膜梭菌; α毒素基因; 基因克隆; 核苷酸序列;
D O I
10.16519/j.cnki.1004-311x.2003.04.001
中图分类号
S852.61 [病原细菌];
学科分类号
090601 ;
摘要
利用聚合酶链式反应 (PCR)技术 ,从A型产气荚膜梭菌染色体基因组中扩增了 1.2kb的α毒素基因。通过T4 DNA连接酶 ,将纯化的PCR产物与载体pGEM -T连接 ,转化至受体菌JM10 9中。经NcoI EcoRI和BamHI EcoRI双酶切分析 ,证明重组质粒pXCPA0 2中含有A型产气荚膜梭菌α毒素全基因。经核苷酸序列分析 ,明确了克隆的α毒素基因在重组质粒中的连接向位且核苷酸序列是正确的。
引用
收藏
页码:1 / 2
页数:2
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