铂类络合物引起的DNAO6-AGT的耗竭及染色体损伤

被引：2

作者：

王龙贵

刘晓梅

范培东

籍秀娟

机构：

[1] 中国医学科学院药物研究所,中国医学科学院药物研究所,中国医学科学院药物研究所,中国医学科学院药物研究所北京,北京,北京,北京

来源：

药学学报 | 1991年 / 08期

关键词：

宁辛铂; Mer细胞; DNA鸟嘌呤O6-烷基转移酶; 染色体损伤;

D O I：

10.16438/j.0513-4870.1991.08.001

中图分类号：

学科分类号：

摘要：

本文测定了KB,CHL,HL-60和L1210细胞DNA鸟嘌呤O6-烷基转移酶（O6-AGT）活性。结果表明,KB细咆有较高的O6-AGT活性,系Mer+细胞。而CHL,HL-60和L1210细胞的O6-AGT活性低,属于Mer-细胞。在此基础上我们观察了顺铂（DDP）、宁辛铂（樟脑胺氯乙酸铂,CCP）和碳铂（JM-8）对KB（Mer+）细胞O6-AGT的影响及Mer+和Mer-细胞的杀伤及微核的诱发作用。结果表明,三种络合物在等毒性浓度下对O6-AGT耗竭程序是CCP>DDP>JM-8,但这种耗竭与杀细胞作用无明显相关性而与微核诱发作用相关。此结果提示铂类络合物对DNA鸟嘌呤O6的损伤可能是其致痛致突变的原因之一。

引用

页码：561 / 566

页数：6

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