亚低温对大鼠脑缺血再灌注损伤后Bcl-2、Bax和C-fos蛋白表达及损伤神经细胞凋亡的影响

被引：7

作者：

吴国祥

李承晏

李涛

李晓清

机构：

[1] 武汉大学人民医院神经内科

[2] 武汉大学人民医院心内科

来源：

中华物理医学与康复杂志 | 2002年 / 10期

关键词：

亚低温; 脑缺血再灌注损伤; Bcl-2; Bax; C-fos; 凋亡;

D O I：

暂无

中图分类号：

R743.05 [];

学科分类号：

1002 ;

摘要：

目的　观察亚低温对大鼠脑缺血再灌注损伤后Bcl-2、Bax和C fos蛋白表达及损伤神经细胞凋亡的影响。方法　采用大鼠局灶性脑缺血再灌注损伤模型 ,大脑中动脉阻塞 2h ,再灌注损伤 6h ,用原位缺口末端标记 (TdtmediateddUTP -biotinnickendlabeling ,TUNEL)法和免疫组织化学法分别检测假手术组、对照组和亚低温组的凋亡细胞百分率和Bcl-2、Bax和C -fos蛋白表达水平。结果　亚低温组Bcl -2蛋白表达水平为 (63 .48± 0 .47) % ,较对照组显著升高 (P <0 .0 5 ) ,而Bax和C -fos蛋白表达水平和凋亡细胞百分率分别为 (6.13± 0 .93 ) %和 (5 .0 9± 0 .2 1) % ,明显低于对照组 (P <0 .0 5 )。结论　亚低温下调大鼠脑缺血再灌注损伤后Bax和C -fos蛋白表达水平和上调Bcl -2蛋白表达水平 ,可能与其抗损伤神经细胞凋亡作用有关。

引用

页码：32 / 34

页数：3

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