聚合酶链反应对输血后丙型肝炎的诊断研究

被引：14

作者：

周荣

梁炽森

骆抗先

机构：

[1] 南方医院传染病科,南方医院传染病科,南方医院传染病科

来源：

第一军医大学学报 | 1991年 / 03期

关键词：

聚合酶链反应; 丙型肝炎病毒核酸;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

为研究丙型肝炎病毒血症的诊断,以三组与病毒不同区段互补的引物进行反转录巢式聚合酶链反应检测输血后肝炎17例(急性期14例)。按日本毒株的引物检出35％,按非编码区序列的引物检出76％的HCV RNA,两组引物共检出94％,而按Chiron原型的引物检测6例均阴性。HCV多变异,且血清水平极低,用几组不同保守区段的引物,进行巢式PCR,可以获得较高的检出率。

引用

页码：181 / 184

页数：4

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