抗氧化作用可能是人参皂甙Rg1抗细胞凋亡的机制

被引：15

作者：

方芳

陈晓春

朱元贵

机构：

[1] 福建医科大学附属协和医院

来源：

中国临床药理学与治疗学 | 2002年 / 05期

关键词：

人参皂甙; MPP+; SHSY5Y细胞; 细胞凋亡;

D O I：

暂无

中图分类号：

R965 [实验药理学];

学科分类号：

摘要：

目的 :探讨人参皂甙Rg1对MPP+诱导SHSY5Y细胞凋亡的保护作用及其可能的机制。方法 :用吖啶橙溴化乙锭染色观察SHSY5Y细胞凋亡率 ,流式细胞仪检测线粒体跨膜电位及细胞内活性氧 (ROS)水平 ,WesternBlot法检测bcl 2、bax蛋白表达水平。结果 :经 10 μmol·L- 1Rg1预处理后 ,MPP+诱导的SHSY5Y细胞凋亡受到明显的抑制 ,虽然线粒体跨膜电位无明显改变 ,但细胞内ROS下降 ,而bcl 2蛋白表达水平增加 ,bax蛋白表达水平减少。结论 :Rg1可抑制MPP+诱导的SHSY5Y细胞凋亡 ,其作用机制可能是通过清除ROS、调节bcl 2、bax蛋白表达水平起作用。

引用

页码：412 / 416

页数：5

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