The volatile oil was extracted from leaf of Cratoxylum formosum(Jack) Dyer subsp.pruniflorum(Kurz) Gogelin in Xishuangbanna of Yunnan Province by steam distillation and its constituents were analyzed by GC-MS technique.The results show that 84 peaks areseparated fromthe volatile oiland 70 compoundsare identified,which represents 96.91% of total content.Sesquiterpenoids are the major chemical constituents in the volatile oil,accounted for 86.01% of total content.The major compounds are caryophyllene(23.99%),α-cedrene(10.57%),curcumene(8.75%) and α-caryophyllene(8.57%),etc.The results provide basic data for comprehensive utilization of C.formosum subsp.pruniflorum.