产生抗单纯疱疹病毒单克隆抗体杂交瘤细胞系的建立

被引：11

作者：

高谦

沈茜

汪美先

机构：

[1] 第四军医大学微生物学教研室

来源：

解放军医学杂志 | 1985年 / 02期

关键词：

杂交瘤细胞系; 克隆化; 阳性克隆; 冻存; McAb; 单克隆抗体; HSV;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

用HSV-1 SM44株免疫C×S/1小鼠的脾细胞与小鼠骨髓瘤细胞系Sp2/O融合,经HAT选择培养,细胞融合率为100％。ELISA筛选后获得53孔产生抗HSV抗体的杂交瘤阳性孔。对1A12,2A8,1G8,1D10,2C5 5株细胞系用有限稀释法做2～5次克隆化,阳性克隆率均达到100％。杂交瘤细胞系经连续传代培养4个月,仍能稳定产生单克隆抗体,冻存的细胞系经复苏后亦能稳定产生抗体。应用ELISA及IF试验证明,5种单克隆抗体均与HSV-1和HSV-2标准株呈特异性反应。ELISA测定,杂交瘤培养上清抗体效价为10-2～10-3,制备免疫腹水效价为10-4～10-7。5种单克隆抗体与EBV,CMV,JEV和HFRSV无交叉反应。

引用

页码：91 / 94

页数：4

共 7 条

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