党参多糖对大鼠脑缺血再灌注脑组织氧自由基和Beclin-1表达的影响

被引：27

作者：

陈文彬 ^{[1
]}

唐瑛 ^{[2
]}

刘李娜 ^{[2
]}

焦勇 ^{[2
]}

机构：

[1] 上海海洋大学食品学院

[2] 海军医学研究所科技处

来源：

华南国防医学杂志 | 2015年 / 29卷 / 12期

关键词：

缺血再灌注; 氧化应激; 自噬; Beclin-1;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的观察党参多糖对大鼠脑缺血再灌注损伤的保护作用,并探讨其可能作用机制。方法改良大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)法制备大鼠局灶性脑缺血模型,2、3、5-氯化三苯基四氮唑(2,3,5-triphenyltetrazolium chloride,TTC)染色法检测大鼠脑梗死面积;化学比色法检测脑组织中丙二醛(malondialdehyde,MDA)含量、超氧化物岐化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力;蛋白免疫印迹法检测自噬特异性基因Beclin-1的表达变化。结果与模型组比较,党参多糖可减小脑梗死面积(30.17±10.11、25.63±10.59),增加SOD(48.36±2.52、56.29±3.41)和GSH-Px(4.33±0.18、5.21±0.11)活性,降低MDA含量(0.13±0.04、0.11±0.02)(P<0.01),抑制Beclin-1表达(0.47±0.04、0.42±0.05)(P<0.05)。结论党参多糖对脑缺血再灌注损伤具有一定的保护作用,可能是通过减轻氧化应激及抑制自噬基因Beclin-1表达而达到的。

引用

页码：886 / 888+923 +923

页数：4

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