糖诱导桃果实园片果实苯丙氨酸解氨酶活性上升和酚类物质积累的研究

被引:28
作者
邓科 [1 ]
孔维府 [1 ,2 ]
战吉宬 [1 ,3 ]
王秀芹 [1 ,3 ]
黄卫东 [1 ,3 ]
机构
[1] 中国农业大学食品科学与营养工程学院
[2] 中国农业大学烟台研究院食品与葡萄酒学院
[3] 中国农业大学葡萄酒科技发展中心
关键词
桃果实; 糖; 苯丙氨酸解氨酶; 酚类物质; 蛋白免疫印迹分析;
D O I
暂无
中图分类号
S662.1 [桃];
学科分类号
090201 [果树学];
摘要
为了探讨OPPP途径与苯丙烷类代谢途径的关系,使用G6PDH的底物和产物以及蔗糖温育分别处理桃果肉圆片,测定了苯丙烷类代谢的关键酶——苯丙氨酸解氨酶的活性变化,以及酚类物质含量的变化。试验结果显示,葡萄糖-6-磷酸、6-磷酸葡萄糖酸、葡萄糖和蔗糖都可以提高PAL活性,相应的总酚和总类黄酮含量也明显增加。而葡萄糖的类似物,2-脱氧葡萄糖的效应和对照相似,对PAL活性和总酚、总类黄酮的含量影响很小。
引用
收藏
页码:162 / 167
页数:6
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