半胱胺对肝细胞代谢油酸过程中ROS生成和ATPase活性的影响

被引：6

作者：

郑建良

施用晖

杨婷婷

乐国伟

机构：

[1] 江南大学食品学院食品科学与安全教育部重点实验室

来源：

中国组织化学与细胞化学杂志 | 2007年 / 03期

关键词：

半胱胺; 油酸; 自由基; ATP酶;

D O I：

暂无

中图分类号：

R96 [药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的研究了半胱胺(CS)对油酸(OA)代谢过程中自由基(ROS)生成和ATP酶(ATPase)活性的影响。方法通过培养体外小鼠原代肝细胞,添加不同浓度的OA作用24h,通过luminol化学发光法,TBA法、DTNB法、无机磷比色法分别测定ROS、丙二醛(MDA)、谷胱甘肽(GSH)和Na+-K+-ATP酶活性;选取OA(0,150,250μM/L)三个梯度,添加CS(0,50,100,150,200,250,300,350,400,450,500μM/L),测定相同的指标。结果添加OA大于150μM/L时,ROS、MDA生成量急剧升高,ATPase活性显著降低(P<0.05)。添加CS能提高GSH,减少ROS和MDA的生成,提高ATPase的活性,且作用的效果显著(P<0.05)。但大量添加CS(350-500μM/L)则效果相反。结论大量添加OA会引起肝细胞氧化应激;添加CS能清除ROS,提高ATPase活性;并伴随OA添加量的增加,CS的最佳需要量也增加。

引用

页码：325 / 329

页数：5

共 9 条

[1]

Assay ofshort-chain acyl coenzyme A intermediates in tissue ex-tracts by high-pressure liquid chromatography. Corkey BE,Brandt M,Williams RJ,et al. Analytical Biochemistry . 1981

[2]

Non-alcoholicsteatohepatitis:review of a growing medical problem. Sligte K,Bourass I,Sels JP,et al. European Journal of Internal Medicine . 2004

[3]

Cysteam-ine increases expression and activity of H+-K+-ATPaseof gastric mucosal cells in weaning piglets. Zhi-Min Shi,Gai-Mei Du,Xi-Hui Wei,et al. World Journal of Gastroenterology . 2005

[4]

Mitochondrialoxidative stress is modulated by oleic acid via an epider-mal growth factor receptor-dependent activation of glu-tathione peroxidase. Duval C,AugéN,Frisach MF,et al. Biochemical Journal . 2002

[5]

Glutathione pathways in thebrain. Dringen R,Hirrlinger J. Biological Chemistry . 2003

[6]

Utility ofthe Nitroblue Tetrazolium Reduction Test for Assess-ment of Reactive Oxygen Species Production by SeminalLeukocytes and Spermatozoa. Esfandiari N,Sharma RK,Saleh RA,et al. Journal of Andrology . 2003

[7]

Reactive oxygen species incell signaling. Thannickal VJ,Fanburg BL. American Journal of Physiology Lung Cellular and Molecular Physiology . 2000

[8]

Fattyacids as natural uncouplers preventing generation of O2and H2O2by mitochondria in the resting state. Korshunov SS,Korkina OV,Ruuge EK,et al. FEBS Letters . 1998

[9]

Activa-tion of epithelial growth factor receptor pathway by un-saturated fatty acids. Vacaresse N,Lajoie-Mazenc I,Auge N,et al. Circulation Research . 1999

← 1 →