齿瓣石斛的位点特异性PCR鉴别

被引：28

作者：

丁小余

徐珞珊

王峥涛

徐红

周开亚

机构：

[1] 中国药科大学中药学院生药学研究室,中国药科大学中药学院生药学研究室,中国药科大学中药学院生药学研究室,中国药科大学中药学院生药学研究室,南京师范大学生命科学学院江苏南京,南京师范大学生命科学学院,江苏南京,江苏南京,江苏南京,江苏南

来源：

药学学报 | 2002年 / 11期

关键词：

齿瓣石斛; 位点特异性PCR; 鉴别;

D O I：

10.16438/j.0513-4870.2002.11.015

中图分类号：

R282.5 [药材鉴定];

学科分类号：

摘要：

目的　设计出齿瓣石斛的位点特异性鉴别引物 ,仅通过PCR就能完成对齿瓣石斛真伪进行准确鉴别。方法　根据齿瓣石斛及其他枫斗类、黄草类石斛的rDNAITS序列数据库 ,设计了齿瓣石斛的位点特异性PCR鉴别引物JB Chiban 0 1S和JB Chiban 0 1X。然后 ,对 38种石斛属植物模板DNA进行了PCR扩增 ,阳性者即为齿瓣石斛正品。结果　当退火温度设定为 5 8℃时 ,只有齿瓣石斛的模板DNA能被扩增出来 ,而其他的 37种石斛属植物均为阴性。该鉴别反应重复性好 ,已在鉴别齿瓣石斛时发挥重要作用。结论　运用位点特异性鉴别引物能成功地对齿瓣石斛进行PCR鉴别 ,与DNA测序鉴别方法相比 ,位点特异性PCR具有高效、准确、简便、省时等优点。

引用

页码：897 / 901

页数：5

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