缺血再灌注大鼠脑内MMP-2、MMP-9与血脑屏障的关系

被引：9

作者：

徐群

张毅

苏敏

苗玲

机构：

[1] 上海交通大学医学院附属仁济医院神经内科

来源：

中风与神经疾病杂志 | 2006年 / 02期

关键词：

基质金属蛋白酶; 再灌注损伤; 血脑屏障; 脑缺血;

D O I：

暂无

中图分类号：

R743.3 [急性脑血管疾病（中风）];

学科分类号：

摘要：

目的明确脑缺血再灌注后MMP-2、MMP-9与血脑屏障的关系。方法健康雄性SD大鼠54只,线栓法制作大鼠大脑中动脉缺血再灌注模型。应用含明胶的定量酶谱技术检测再灌注3h到7d内MMP-2、MMP-9的表达,用甲酰胺浸泡法检测大鼠脑内EB含量。结果 (1)缺血再灌注24h后,大鼠缺血侧脑内MMP-9表达明显升高,48h到达顶峰,4d后显著下降,7d后水平更低。24h组和48h组与其它各组相比,有显著性差异(P<0．05)。 0MMP-2缺血再灌注48h后明显升高,7d到达高峰,7d组大鼠缺血侧脑内MMP-2水平与3h组和24h组相比,有显著性差别(P<0．01)。(2)再灌注24h大鼠缺血侧脑内EB含量最高,显著高于3h及7d组(P<0．05),但与48h无显著差别(P>0．05)。结论缺血再灌注后出现了MMP-2、MMP-9和血脑屏障的动态变化,血脑屏障损伤与MMP-9 的升高相关,而与MMP-2关系不大。

引用

收藏

页码：146 / 148

页数：3

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