浊点系统中青霉素水解物系的分配及固定化青霉素酰化酶的稳定性

被引：2

作者：

郭雅静 ^{[1
]}

王志龙 ^{[1
]}

包达 ^{[2
]}

齐瀚实 ^{[1
]}

机构：

[1] 上海交通大学药学院

[2] 上海海湃生物科技有限公司

来源：

化工学报 | 2006年 / 06期

关键词：

浊点系统; 青霉素水解; 青霉素酰化酶; 分配; 稳定性;

D O I：

暂无

中图分类号：

TQ465.1 [青霉素及其衍生物];

学科分类号：

100705 ;

摘要：

Enzymatic hydrolysis of penicillin G(Pen G)into phenylacetic acid(PAA)and 6-aminopenicillanic acid(6-APA)suffers not only substrate inhibition and non-competitive inhibition by 6-APA,but also competitive inhibition by PAA.In the industrial process,PAA is generally eliminated by addition of aqueous ammonia to form soluble ammonium salt,which generates industrial waste.Extractive biocatalysis is a potential method to overcome the above obstacles and might be even directly used in enzymatic hydrolysis of penicillin G from fermentation broth.The partitioning behavior of Pen G hydrolysis reactants and the stability of immobilized penicillin acylase in cloud point system,a novel extractive bioreaction system,were determined.It suggests that cloud point system might be a novel extractive bioreaction system for the industrial process of penicillin hydrolysis.

引用

页码：1422 / 1425

页数：4