夏玉米根系密集区与行间N2O浓度及与氨氧化细菌和反硝化细菌数量的关系

被引:26
作者
保琼莉
巨晓棠
机构
[1] 中国农业大学资源与环境学院植物营养系农业部植物营养学重点实验室
关键词
夏玉米; 根系密集区; N2O; amoA基因拷贝数; nirK/nirS基因拷贝数;
D O I
暂无
中图分类号
S513 [玉米(玉蜀黍)];
学科分类号
090104 [作物信息科学与技术];
摘要
采用硅胶管法和微生物分子生态学方法对华北平原典型旱地土壤夏玉米根系密集区与行间土壤产生的N2O及与氨氧化细菌和反硝化细菌数量的关系进行了研究。结果表明:夏玉米三叶期和十叶期的施肥灌水措施以及超过15 mm左右的降雨均显著促进了土壤N2O的产生。夏玉米行间产生的N2O略高于根系密集区,但二者未达显著差异水平。相关分析表明,影响土壤N2O产生的主要环境因子为土壤空隙含水量(WFPS)及土壤NO-3-N含量,二者决定系数分别为0.79和0.51。N2O浓度与氨氧化细菌amoA基因拷贝数及反硝化细菌nirS基因拷贝数之间的显著正相关关系(决定系数分别达0.55和0.62)表明土壤中N2O的产生与氨氧化细菌和nirS基因反硝化细菌数量的增加有关。表明在该试验条件下,夏玉米根系生长对根系密集区土壤中氮和水的消耗以及氨氧化细菌和反硝化细菌数量的变化并未引起根系密集区和玉米行间N2O浓度的显著差异,即在田间布设N2O观测箱时,采样箱是否覆盖作物根系或采样箱覆盖作物根系的多少对N2O排放量的测定结果没有显著性影响。
引用
收藏
页码:1156 / 1165
页数:10
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