芍药苷对大鼠局灶性脑缺血再灌注脑血流量及PGI/TXA平衡的影响

被引：38

作者：

饶梦琳

唐蜜

何锦悦

董志

机构：

[1] 重庆医科大学药学院生化与分子药理研究室

来源：

药学学报 | 2014年 / 01期

关键词：

芍药苷; 脑缺血再灌注; 激光多普勒血流仪; 前列腺素I2; 血栓素A2;

D O I：

10.16438/j.0513-4870.2014.01.008

中图分类号：

R285.5 [中药实验药理];

学科分类号：

摘要：

探讨芍药苷对大鼠局灶性脑缺血再灌注脑血流量变化及PGI2/TXA2平衡的影响。取72只雄性SD大鼠,随机分为假手术组、I/R模型组、芍药苷(10、20及40 mg·kg-1)处理组以及尼莫地平组。线栓法制作大鼠局灶性脑缺血(90 min)/再灌注(24 h)模型。观察芍药苷对神经功能缺损评分及梗死体积的影响,采用激光多普勒血流仪监测给药前后大鼠缺血侧局部脑血流量的动态变化,免疫组化法检测缺血侧海马CA1区COX-2的表达;ELISA法测血清中血栓素A2(TXA2)和前列腺素I2(PGI2)含量并计算PGI2/TXA2比值。结果表明,芍药苷处理组较I/R模型组能显著改善神经功能缺损症状,缩小梗死体积,增加缺血侧大脑局部血流量,抑制COX-2阳性表达,降低TXA2含量,提高PGI2含量及PGI2/TXA2比值。结果提示,芍药苷对局灶性缺血大脑产生神经保护作用的机制可能与其改善缺血侧大脑血流供应,调节PGI2/TXA2平衡有关。

引用

页码：55 / 60

页数：6

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