不同施肥处理对毛竹根际微生物影响及其PCR-DGGE分析

被引:6
作者
刘玮 [1 ]
邓光华 [1 ]
张嘉超 [2 ]
杨清培 [1 ]
机构
[1] 江西农业大学
[2] 湖南大学
关键词
毛竹; 根际微生物; PCR-DGGE分析; 施肥处理;
D O I
10.13759/j.cnki.dlxb.2011.05.036
中图分类号
S795.7 [刚竹];
学科分类号
0829 ; 0907 ;
摘要
采用PCR-DGGE技术对不同施肥处理毛竹根际微生物多样性特征进行研究。结果表明:采用改进的蛋白酶K-CTAB法提取的毛竹土壤DNA经PCR扩增的产物经DGGE检测后得到的电泳条带清晰且分离效果好,可以明显反映出毛竹根际微生物多样性的变化。根际微生物种群组成特征受施肥量、肥料种类影响很大,施肥能够增加毛竹根际微生物的多样性指数,在同等氮、磷养分情况下,钾肥的加入会对毛竹根际微生物种群组成产生很大的影响,并且根际微生物多样性有随钾肥施用量增加而升高的趋势。
引用
收藏
页码:50 / 53
页数:4
相关论文
共 31 条
[11]  
世界竹藤[M]. 辽宁科学技术出版社 , 江泽慧主编, 2002
[12]  
普通生态学[M]. 复旦大学出版社 , 郑师章等编著, 1994
[13]   Succession and phylogenetic profile of eukaryotic communities in the composting process of rice straw estimated by PCR-DGGE analysis [J].
Cahyani, VR ;
Matsuya, K ;
Asakawa, S ;
Kimura, M .
BIOLOGY AND FERTILITY OF SOILS, 2004, 40 (05) :334-344
[14]   Structure and function of the soil microbial community in a long-term fertilizer experiment [J].
Marschner, P ;
Kandeler, E ;
Marschner, B .
SOIL BIOLOGY & BIOCHEMISTRY, 2003, 35 (03) :453-461
[15]   Bacterial activity and community composition in stream water and biofilm from an urban river determined by fluorescent in situ hybridization and DGGE analysis [J].
Araya, R ;
Tani, K ;
Takagi, T ;
Yamaguchi, N ;
Nasu, M .
FEMS MICROBIOLOGY ECOLOGY, 2003, 43 (01) :111-119
[16]   Microbial diversity and activity along the forefields of two receding glaciers [J].
Sigler, WV ;
Zeyer, J .
MICROBIAL ECOLOGY, 2002, 43 (04) :397-407
[17]  
Soil microbial community responses to dairy manure or ammonium nitrate applications[J] . A.D. Peacock,M.D. Mullen,D.B. Ringelberg,D.D. Tyler,D.B. Hedrick,P.M. Gale,D.C. White.Soil Biology and Biochemistry . 2001 (7)
[18]   Soil and plant specific effects on bacterial community composition in the rhizosphere [J].
Marschner, P ;
Yang, CH ;
Lieberei, R ;
Crowley, DE .
SOIL BIOLOGY & BIOCHEMISTRY, 2001, 33 (11) :1437-1445
[19]   Microbial distribution and function across wheat rhizosphere with oxamide and ammonium sulfate as N sources [J].
Miah, MY ;
Kanazawa, S ;
Chiu, CY ;
Hayashi, H ;
Chino, M .
SOIL SCIENCE AND PLANT NUTRITION, 2000, 46 (04) :787-796
[20]   Digitization of DGGE (denaturing gradient gel electrophoresis) profile and cluster analysis of microbial communities [J].
Zhang, T ;
Fang, HHP .
BIOTECHNOLOGY LETTERS, 2000, 22 (05) :399-405