抑制p38 MAPK信号通路对海马神经元毒性损伤的保护作用

被引：8

作者：

刘智良

徐如祥

机构：

[1] 第一军医大学珠江医院全军神经医学研究所神经外科

[2] 第一军医大学珠江医院全军神经医学研究所神经外科广东广州

[3] 广东广州

来源：

中华神经医学杂志 | 2003年 / 03期

关键词：

海马/神经元; 红藻氨酸/药物作用; p38 MAPK/SB203580; 细胞膜/超微结构; 原子力显微镜;

D O I：

暂无

中图分类号：

R742.1 [癫痫];

学科分类号：

1002 ;

摘要：

目的通过观察细胞表面形态的三维构像变化,探讨抑制p38 MAPK通路对减轻红藻氨酸(KA)毒性作用引起大鼠海马神经元所造成损害的作用和机制。方法原代培养10 d的海马神经元给予SB203580(0.2μmol/L),p38MAPK特异性抑制剂)预处理.30min后再予不同浓度(0μmol/L,25μmol/L和250μmol/L)KA分别作用10 min和100 min,利用原子力显微镜(AFM)对细胞表面结构进行纳米级水平扫描和观测。结果正常海马神经元表面光滑,起伏均匀、规律;KA作用后神经元呈退行性改变,表现为胞体肿胀,胞膜表面粗糙,出现隆起和“孔洞”样胞膜破裂结构,并且其变化程度分别与作用时间和KA浓度呈量-效关系;预先给予SB203580处理,以上变化有所减轻。结论KA毒性作用后海马神经元胞膜表面超微结构所产生明显变化;p38MAPK信号通路参与这种损害过程;抑制该信号转导通路,对海马神经元的毒性损害起一定保护作用。

引用

页码：194 / 198

页数：5

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