激活脊髓小电导钙离子激活钾通道可抑制小鼠吗啡痛觉过敏

被引：5

作者：

朱骏生 ^{[1
]}

章功良 ^{[2
]}

杜雷 ^{[1
]}

姬宁宁 ^{[1
]}

黄思婷 ^{[1
]}

张咏梅 ^{[1
]}

花嵘 ^{[1
,3
]}

机构：

[1] 徐州医科大学江苏省麻醉学重点实验室江苏省麻醉与镇痛应用技术重点实验室

[2] 安徽医科大学基础医学院

[3] 徐州医科大学附属医院急救中心

来源：

中国药理学通报 | 2017年 / 04期

关键词：

SK通道; 1-EBIO; 吗啡; 痛觉过敏; 脊髓; 小鼠;

D O I：

暂无

中图分类号：

R965 [实验药理学];

学科分类号：

100706 [药理学];

摘要：

目的探究脊髓小电导钙离子激活钾通道(small conductance Ca2+-activated K+channels,SK通道)激活后对小鼠吗啡所致痛觉过敏的影响。方法选用♂C57BL6/N小鼠,建立吗啡痛觉过敏模型,鞘内注射SK通道激活剂1-EBIO后,测量小鼠热甩尾潜伏期（tail withdrawal latency,TWL）,机械缩足阈值（mechanical withdrawal threshold,MWT）和内脏痛阈的变化。结果吗啡痛觉过敏模型小鼠与生理盐水对照组小鼠相比,热甩尾潜伏期、机械缩足阈值和内脏痛阈均降低;而鞘内注射SK通道激活剂1-EBIO后,与给药前相比,小鼠的痛阈热甩尾潜伏期,机械缩足阈值和内脏痛阈均升高;吗啡模型组小鼠的脊髓SK2膜蛋白表达量较对照组明显降低,而给予1-EBIO后,脊髓SK2膜蛋白表达量较模型组明显升高。结论脊髓SK通道参与小鼠吗啡引起的痛觉过敏。

引用

页码：547 / 551

页数：5

共 8 条

[1]

5-羟色胺5-HT_(2C)受体激动剂抑制吗啡依赖小鼠纳洛酮诱导的戒断行为[J] 吴县;江勤;庞刚;刘欢;陶欣荣;董六一;章功良; 中国药理学通报 2015, 04

[2]

Opioid-Induced Hyperalgesia (OIH): A Real Clinical Problem or Just an Experimental Phenomenon?[J] Elon Eisenberg;Erica Suzan;Dorit Pud Journal of Pain and Symptom Management 2015,

[3]

Loss of NR1 Subunit of NMDARs in Primary Sensory Neurons Leads to Hyperexcitability and Pain Hypersensitivity: Involvement of Ca2+-Activated Small Conductance Potassium Channels[J] Promila Pagadala;Chul-Kyu Park;Sangsu Bang;Zheng-Zhong Xu;Rou-Gang Xie;Tong Liu;Bao-Xia Han;W. Daniel Tracey;Fan Wang;Ru-Rong Ji Journal of Neuroscience 2013,

[4]

Small-Conductance Ca<sup>2+</sup>-Activated K<sup>+</sup> Channels: Form and Function[J] John P. Adelman;James Maylie;Pankaj Sah Annual Review of Physiology 2012,

[5]

Functions and Modulation of Neuronal SK Channels[J] E. S. Louise Faber Cell Biochemistry and Biophysics 2009,

[6]

Morphine hyperalgesia in mice is unrelated to opioid activity; analgesia; or tolerance: Evidence for multiple diverse hyperalgesic systems[J] Aaron Juni;Gad Klein;Benjamin Kest Brain Research 2005,

[7]

Potassium channels and pain: present realities and future opportunities[J] European Journal of Pharmacology 2004,

[8]

The distribution of small and intermediate conductance calcium-activated potassium channels in the rat sensory nervous system[J] L.C. Mongan;M.J. Hill;M.X. Chen;S.N. Tate;S.D. Collins;L. Buckby;B.D. Grubb Neuroscience 2004,

← 1 →