Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor

被引：2

作者：

杨云梅

曹红翠

徐哲荣

陈晓明

机构：

[1] First Affiliated Hospital

[2] College of Medical Science

[3] Zhejiang University

[4] Zhejiang University Hangzhou

[5] China

[6] Hangzhou

来源：

Journal of Zhejiang University Science | 2004年 / 06期

关键词：

Platelet activating factor; Reversed phase high-performance liquid chromatographic technique; Coronary heart disease; Cerebral infarction;

D O I：

暂无

中图分类号：

R446.119 [];

学科分类号：

100208 ;

摘要：

Objective: To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart disease. Methods: To measure PAF levels in 100 controls, 23 elderly patients with cerebral infarction and 65 cases with coronary heart disease by reversed phase high-performance liquid chromatographic technique (rHPLC). Results: rHPLC is more convenient, sensitive, specific, and less confusing, compared with bio-assay. The level of plasma PAF in patients with cerebral infarction was higher than that in the controls (P<0.01), and in patients with coronary heart disease. Conclusion: Detection of PAF with rHPLC is more reliable and more accurate. The new assay has important significance in PAF research.

引用

页码：112 / 116

页数：5