氯胺酮对He-Ne激光照射促人正常皮肤成纤维细胞增殖作用的影响

被引：11

作者：

李燕

角建瓴

刘承宜

洪岸

刘颂豪

机构：

[1] 华南师范大学激光运动医学实验室

[2] 暨南大学中医系

[3] 暨南大学生物工程研究所

[4] 华南师范大学信息光电子学院广东广州

[5] 广东广州

[6] 华中科技大学教育部生物医学光子学重点实验室和激光技术国家重点实验室

[7] 湖北武汉

来源：

中国激光 | 2004年 / 04期

基金：

广东省自然科学基金;

关键词：

激光技术; 成纤维细胞; 低强度激光; 氯胺酮;

D O I：

暂无

中图分类号：

R454 [物理疗法、自然疗法];

学科分类号：

100215 ;

摘要：

针对动物模型伤口的光生物调节作用 ,用He Ne激光对人正常皮肤成纤维细胞增殖的促进作用作为细胞模型 ,研究了麻醉药氯胺酮的影响。用MTT法测定细胞增殖作用 ,分别研究了 1 5 6mW/cm2 He Ne激光 ( 0 ,10 ,30 ,10 0和 30 0s照射 )对人正常皮肤成纤维细胞的增殖作用和氯胺酮 ( 0 ,0 6 ,1 2和 2 0 μg/mL)预处理对He Ne激光 ( 30 0s照射 )细胞增殖作用的影响。氯胺酮 ( 0 6 ,1 2 ,2 0 μg/mL)预处理能抑制 4 6 8 7mJ/cm2 He Ne激光促人正常皮肤成纤维细胞增殖的作用。这说明动物实验中用于麻醉的药物可能对激光促进伤口愈合的实验结果有影响。

引用

页码：509 / 512

页数：4

共 13 条

[1]

The effect of low-energy infrared laser radiation on wound healing in rats. G. Anneroth,G. Hall,H. Ryden et al. Br. J. Oral . and Maxillofac Surg . 1988

[2]

Re-examination and further development of precise and rapid dye method for measuring cell growth/cell kill. M. B. Hansen,S. E. Nielsen,K. Berg. Journal of Immunological Methods . 1989

[3]

A suitable mode for wound healing: how many times are we to stumble over the same block. T. I. Karu. Lasers in Surgery and Medicine . 1999

[4]

Comparison of cold-quartz ultraviolet, low-energy laser, a occlusion in wound healing in a swine model. J. R. Basford,H. O. Hallman,C. G. Sheffield et al. Archives of Physical Medicine and Rehabilitation . 1986

[5]

Effect of low-power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts. A. N. Pereira,C. P. Eduardo,E. Matson et al. Lasers in Surgery and Medicine . 2002

[6]

Helium-neon laser irradiation at fluence of 1, 2, and 4 J/cm2 failed to accelerate wound healing as assessed by both wound contracture rate and tensile strength. J. D. F. Allendorf,M. Bessler,J. Huang et al. Lasers in Surgery and Medicine . 1997

[7]

Histologic signatures of thermal injury: applications in transmyocardial laser revascularization and radiofrequency ablation. P. Whittaker,S. M. Zheng,M. J. Patterson et al. Lasers in Surgery and Medicine . 2000

[8]

Low-energy laser therapy: controversies and new research finding. J. R. Basford. Lasers in Surgery and Medicine . 1989

[9]

Stimulatory effect of 660 nm low level laser energy on hypertrophic scar-derived fibroblasts: possible mechanism for increase in cell counts. C. Webb,M. Dyson,W. H. Lewis. Lasers in Surgery and Medicine . 1998

[10]

Effect of low-power density laser irradiation on healing of open skin wound in rats. J. S. Kana,G. Hutschenreiter,D. Haina et al. Archives of Surgery . 1981

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