脐血单个核细胞培养前后神经细胞特有标志物的变化

被引:10
作者
邢莹
鄢文海
刘计荣
龚光明
许燕
张莹
曹孟德
机构
[1] 郑州大学医学院干细胞研究中心
[2] 郑州大学第一附属医院肿瘤科
关键词
脐血细胞; 巢蛋白; 神经丝亚单位M; 微管相关蛋白-2; 神经干细胞;
D O I
10.13705/j.issn.1671-6825.2003.02.021
中图分类号
R329 [人体组织学];
学科分类号
摘要
目的 :探讨脐血单个核细胞培养前后神经细胞特有标志物的表达差异。方法 :采用RT PCR方法对脐血单个核细胞培养前后nestin ,神经丝亚单位M(NF M)及微管相关蛋白 2 (MAP2mRNA)的表达进行检测。结果 :nestin ,NF M及MAP2mRNA在培养前的脐血单个核细胞中表达呈阳性 ,培养后 3者表达增强。结论 :直接分离的脐血单个核细胞中存在着表达神经细胞特有分子的细胞 ,培养后 ,神经元样细胞增多
引用
收藏
页码:200 / 202
页数:3
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