H2O2介导的NO合成参与乙烯诱导的拟南芥叶片气孔关闭

被引：22

作者：

刘国华 ^{[1
]}

侯丽霞 ^{[1
]}

刘菁 ^{[1
]}

刘新 ^{[1
,2
]}

王学臣 ^{[2
]}

机构：

[1] 青岛农业大学生命科学学院

[2] 中国农业大学生物学院植物生理学与生物化学国家重点实验室

来源：

自然科学进展 | 2009年 / 19卷 / 08期

关键词：

拟南芥; 一氧化氮; 过氧化氢; 乙烯; 气孔运动;

D O I：

暂无

中图分类号：

Q945 [植物生理学];

学科分类号：

0903 ;

摘要：

利用药理学实验、激光共聚焦显微技术结合遗传学手段,证明光下乙烯利可诱导拟南芥叶片气孔关闭,且具有时间和剂量效应.乙烯利能够明显增加气孔保卫细胞和叶片的H2O2和NO水平,且H2O2和NO清除剂均明显抑制乙烯利诱导的拟南芥叶片气孔关闭.硝酸还原酶(NR)、NADPH氧化酶和细胞壁过氧化物酶的抑制剂可不同程度抑制乙烯利的作用.乙烯利亦可明显诱导Atnoa1突变体叶片气孔关闭及NO的积累,但对nia1,nia2突变体没有明显影响.清除H2O2可减弱乙烯利对NO含量和NR活性的诱导效应,说明H2O2和NO均参与乙烯诱导的拟南芥叶片气孔关闭,且NO(主要由NR途径合成)可能位于H2O2下游参与调控这一信号通路.

引用

页码：841 / 851

页数：11

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