槐角中的抗癌活性成分

AIM: To study the isoflavonoids and their glycosides from the fruits of Sophora japonica L.and to investigate the anticancer activity in vitro of these compounds.METHOD: The constituents were isolated and purified by chromatographic techniques and their structures were elucidated by physico-chemical evidence and spectra data.The inhibitory activity on cultured human cancer cell lines was determined by MTT assay.RESULTS: Nine compounds were isolated and identified as:genistein（Ⅰ）,sophororicoside（Ⅱ）,genistin（Ⅲ）,sophorabioside（Ⅳ）,genistein 7,4′-di-O-βD-glucoside（Ⅴ）,formononetin（Ⅵ）,ononin（Ⅶ）,daidzin（Ⅷ） and afrormosin （Ⅸ）.Results showed that tumor inhibitory rate of compounds Ⅰ～Ⅲ on(100 Hg·mL-1) A549 reached 82.01%,38.87% and 32.97% respectively,and on BGC-823 reached 91.25%,23.26% and 13.98% respectively.CONCLUSION: Compounds Ⅶ and Ⅷ were isolated from this plant for the first time;compound Ⅸ was isolated from the fruits of the plant for the first time.The study showes that genistein has inhibitory effect on A549 and BGC-823 cell in vitro.