太湖不同湖区真核微型浮游生物基因多样性的研究

被引:21
作者
陈美军
孔繁翔
陈非洲
邢鹏
机构
[1] 中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室
关键词
微食物网; 真核微型浮游生物; DGGE; 18SrDNA克隆文库; 基因多样性;
D O I
10.13227/j.hjkx.2008.03.014
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
用DGGE(denatured gradient gel electrophoresis)和构建18SrDNA克隆文库2种方法对太湖不同湖区的真核微型浮游生物(0.8~20μm)多样性及组成结构进行了研究.DGGE结果表明,不同湖区真核微型浮游生物的DGGE指纹图谱存在明显差异,其中营养水平较低的东太湖和贡湖DGGE条带数最多,分别为23和24,香农多样性指数分别为3.135和3.178,而营养水平较高的梅梁湾和五里湖最少,均为18,香农多样性指数为2.890,表明营养水平较低湖区的多样性高于营养水平较高的湖区.克隆测序结果表明太湖中真核微型浮游生物种类丰富,占优势的主要是一些鞭毛藻、异养鞭毛虫、纤毛虫和真菌,而营养水平不同的梅梁湾、湖心、东太湖中真核微型浮游生物组成明显不同.在营养水平较高的梅梁湾,28.6%的OUT(operational taxonomicunit)属于异养鞭毛虫,另外隐藻、金藻分别占22.9%和14.3%;在湖心,金藻的比例最大,占25.7%,另外比较多的是异养鞭毛虫和隐藻,分别为20.0%和14.3%;而营养水平较低的东太湖各类纤毛虫所占比份最大,为26.8%,异养鞭毛虫较少,仅占4.9%,另外真菌含量较高,占12.2%.
引用
收藏
页码:769 / 775
页数:7
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