铜绿假单胞菌外毒素A的生产、分离纯化和鉴定

被引：8

作者：

梁小兵

庄汉澜

周洁

詹轶群

郭庆福

机构：

[1] 军事医学科学院微生物流行病研究所

来源：

生物技术通讯 | 2000年 / 02期

关键词：

铜绿假单胞菌; 外毒素A; 生产; 纯化; 鉴定;

D O I：

暂无

中图分类号：

Q517 [氨基酸];

学科分类号：

摘要：

通过对培养基组成、种子活化、接种量和培养条件进行优化 ,使铜绿假单胞菌外毒素A(PE)产量达到每毫升 5～ 10 μg和 192小鼠LD50 ,不低于国外报道水平。经二步纯化 ,PE蛋白回收率为33.33% (PE)和 16.67% (LD50 ) ,提纯系数为 4 38.5 (PE)或 2 18.5 (LD50 ) ,SDS PAGE呈现一条带 ,相对分子质量为 660 0 0 ,琼脂糖扩散鉴定与兔抗PE产生一条沉淀线 ,小鼠半数致死量为 0 .16μg ,PE对小鼠成纤维母细胞L92 9有毒性作用 ,能被兔抗PE血清所中和。

引用

页码：107 / 110+114 +114

页数：5

共 12 条

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