番茄溃疡病菌实时荧光PCR快速检测方法研究

被引：8

作者：

吴兴海

邵秀玲

邓明俊

陈长法

厉艳

梁成珠

机构：

[1] 山东出入境检验检疫局

来源：

江西农业学报 | 2007年 / 03期

关键词：

番茄溃疡病菌; 实时荧光PCR; 快速; 检测方法;

D O I：

10.19386/j.cnki.jxnyxb.2007.03.012

中图分类号：

S436.412 [番茄病虫害];

学科分类号：

摘要：

根据番茄溃疡病菌ITS（16S23SrDNA间隔区）基因序列,设计并合成了荧光PCR检测引物（Fan1和Fan2）及特异性检测探针（Fanprobe）,对番茄溃疡病菌和非番茄溃疡病菌的标准菌株实时荧光PCR反应。结果表明,番茄溃疡病菌PCR产物出现强烈的特异杂交信号,而非番茄溃疡病菌均未出现特异荧光信号,证明这对引物及探针具有番茄溃疡病菌鉴定特异性。将番茄溃疡病菌DNA做梯度稀释,测定该检测体系的敏感度,结果表明,此体系可检出52.3 fg/μL的模板。

引用

页码：34 / 36

页数：3

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