实时荧光定量PCR的研究进展及其应用

被引:75
作者
张蓓
机构
[1] 上海第二医科大学附属新华医院上海儿童医学中心实验诊断中心上海
关键词
实时多聚酶链反应(real-time PCR); 荧光; 定量; 临床应用;
D O I
暂无
中图分类号
R346 [];
学科分类号
摘要
多聚酶链反应飞速的发展使其成为了分子生物学实验室重要的工具 ,实时荧光定量PCR(real timequantitativePCR)技术以其敏感性高、重复性好、速度快和污染少使PCR技术又向前迈进了一步。本文对real timeQ PCR技术的原理、五种主要的荧光化学及定量方法作一介绍 ,同时也讨论了此技术存在的不足 ,并对其目前在研究领域中主要的应用进行了概述。
引用
收藏
页码:327 / 329
页数:3
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