IT-ISJ标记及其在陆地棉遗传图谱构建中的应用

被引：12

作者：

郑靓

张正圣

陈利

万群

胡美纯

王威

张轲

刘大军

陈笑

魏新琦

机构：

[1] 西南大学农学与生物科技学院/农业部生物技术与作物品质改良重点实验室

来源：

中国农业科学 | 2008年 / 08期

关键词：

内含子定靶的内含子-外显子拼接位点(IT-ISJ); 陆地棉; 遗传图谱;

D O I：

暂无

中图分类号：

S562 [棉];

学科分类号：

0901 ;

摘要：

【目的】开发可用于植物遗传多样性分析和遗传图谱构建的新型标记。【方法】根据植物结构基因内含子拼接位点的保守序列,设计扩增内含子的内含子-外显子拼接位点(intron targeted intron-exon splice junction,IT-ISJ)标记引物,并用于陆地棉遗传图谱构建。【结果】利用704个IT-ISJ引物组合,对陆地棉高品质品种渝棉1号和多显性基因标记系T586进行多态性筛选,获得49个多态性引物组合,占筛选引物的7.0%。49个多态性引物组合在(渝棉1号×T586)F2:7重组近交系群体270个系中检测到58个位点。以58个IT-ISJ、150个SSR和8个形态标记进行连锁分析,构建的遗传连锁图包括22个连锁群、113个位点(49个IT-ISJ、62个SSR和2个形态标记)。连锁图覆盖714.5cM,占棉花基因组的16.1%,标记间平均长度为6.3cM。【结论】IT-ISJ标记多态性高,可有效地用于陆地棉遗传连锁图谱的构建。

引用

页码：2241 / 2248

页数：8

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