胎鼠海马神经干细胞生物学特性的研究

被引:10
作者
刘辉
杨树源
高永中
张建宁
张文治
机构
[1] 天津医科大学总医院神经外科
[2] 天津市环湖医院 天津
[3] 天津
关键词
神经干细胞; 海马; 碱性成纤维生长因子; 分裂增殖; 分化;
D O I
暂无
中图分类号
R329.28 [];
学科分类号
100101 ;
摘要
目的 研究胎鼠脑组织中海马神经干细胞的自我繁殖特性及多向分化潜能。方法 在碱性成纤维生长因子(FGF-2)作用下从胎鼠脑组织中分离、培养神经干细胞并诱导其分化,然后采用间接免疫荧光和免疫组化技术研究神经干细胞的特性。结果 由胎鼠海马中分离出的神经干细胞在体外分裂增殖形成干细胞团,分化产生神经元和胶质细胞,它们分别对神经上皮干细胞蛋白、神经元特异性烯醇化酶、胶质纤维酸性蛋白等抗原标记呈现阳性。结论 胎鼠脑海马中存在神经干细胞,其具有自我增殖特性和多向分化潜能,在FGF-2的作用下分裂增殖,并分化成神经元、胶质细胞。
引用
收藏
页码:273 / 275+5 +5
页数:4
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