铜型亚硝酸还原酶的电子传递模式及催化机理研究进展

被引：4

作者：

胡朝松

李春强

廖文彬

彭明

机构：

[1] 中国热带农业科学院热带生物技术研究所

来源：

微生物学通报 | 2008年 / 07期

关键词：

铜型亚硝酸还原酶; 电子传递; 催化机理; 生物脱氮;

D O I：

10.13344/j.microbiol.china.2008.07.022

中图分类号：

X703 [废水的处理与利用];

学科分类号：

083002 ;

摘要：

亚硝酸还原酶(NiRs)是反硝化过程中的关键作用酶,它使NO2?转变成NO,减轻了水体中的氮污染。根据辅基的不同,NiRs分为血红素cd1型亚硝酸还原酶(cd1-NiRs)和铜型亚硝酸还原酶(Cu-NiRs)两种。Cu-NiRs呈三聚体结构,每个单体都含有两种类型的铜原子,它们在酶催化过程中起着传递电子的重要作用。在催化过程中,Cu-NiRs中部分残基的结构变化有利于催化反应的进行。此文结合Cu-NiRs的最新研究成果,从整体上对其结构特点、电子传递过程及催化机理等研究作了系统地阐述。

引用

页码：1136 / 1142

页数：7

共 21 条

[1] Thermal stability effects of removing the type-2 copper ligand His306 at the interface of nitrite reductase subunits [J].

Stirpe, Andrea ;

Sportelli, Luigi ;

Wijma, Hein ;

Verbeet, Martin Ph. ;

Guzzi, Rita .

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2007, 36 (07) :805-813

[2] Genomic analysis reveals widespread occurrence of new classes of copper nitrite reductases [J].

Ellis, Mark J. ;

Grossmann, J. Guenter ;

Eady, Robert R. ;

Hasnain, S. Samar .

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2007, 12 (08) :1119-1127

[3]

Greenhouse gas production in a pond sediment: Effects of temperature, nitrate, acetate and season[J] . Johanna Stadmark,Lars Leonardson.Science of the Total Environment . 2007 (1)

[4]

The structure of the Met144Leu mutant of copper nitrite reductase from Alcaligenes xylosoxidans provides the first glimpse of a protein–protein complex with azurin II[J] . Konstantinos Paraskevopoulos,Michael A. Hough,R. Gary Sawers,Robert R. Eady,S. Samar Hasnain.JBIC Journal of Biological Inorganic Chemistry . 2007 (6)

[5] Selenite-reducing capacity of the copper-containing nitrite reductase of Rhizobium sullae [J].

Basaglia, Marina ;

Toffanin, Annita ;

Baldan, Enrico ;

Bottegal, Mariangela ;

Shapleigh, James P. ;

Casella, Sergio .

FEMS MICROBIOLOGY LETTERS, 2007, 269 (01) :124-130

[6] Coordinate synthesis of azurin I and copper nitrite reductase in Alcaligenes xylosoxidans during denitrification [J].

Harris, Roger L. ;

Eady, Robert R. ;

Hasnain, S. Samar ;

Sawers, R. Gary .

ARCHIVES OF MICROBIOLOGY, 2006, 186 (03) :241-249

[7] High resolution structural studies of mutants provide insights into catalysis and electron transfer processes in copper nitrite reductase [J].

Hough, MA ;

Ellis, MJ ;

Antonyuk, S ;

Strange, RW ;

Sawers, G ;

Eady, RR ;

Hasnain, SS .

JOURNAL OF MOLECULAR BIOLOGY, 2005, 350 (02) :300-309

[8] Identification of denitrifying bacteria diversity in an activated sludge system by using nitrite reductase genes [J].

You, SJ .

BIOTECHNOLOGY LETTERS, 2005, 27 (19) :1477-1482

[9]

Heterologous metalloprotein biosynthesis in Escherichia coli : conditions for the overproduction of functional copper‐containing nitrite reductase and azurin from Alcaligenes xylosoxidans[J] . Roger L. Harris,Miguel Prudêncio,S. Samar Hasnain,Robert R. Eady,R. Gary Sawers.J. Synchrotron Rad. . 2005 (1)

[10]

Expression of nitrite reductase in Nitrosomonas europaea involves NsrR, a novel nitrite‐sensitive transcription repressor[J] . Hubertus J. E.Beaumont,Sylvia I.Lens,Willem N. M.Reijnders,Hans V.Westerhoff,Rob J. M.Van Spanning.Molecular Microbiology . 2004 (1)

← 1 2 3 →