苯那普利对糖尿病大鼠肾皮质p21CIP1蛋白表达的调节及其机制

被引:16
作者
林善锬
吴永贵
余毅
周江华
机构
[1] 上海医科大学华山医院肾内科!
关键词
苯那普利; 糖尿病肾病; p21CIP1蛋白; 转化生长因子β;
D O I
暂无
中图分类号
R587.1 [糖尿病];
学科分类号
摘要
目的 研究苯那普利对糖尿病大鼠肾皮质p21 C I P1 蛋白表达 的调节及其机制。方法大鼠随机分单侧肾切除对照组、糖尿病组及糖尿病苯那普利(10 m g·kg - 1 ·日- 1 ,灌胃) 治疗组。应用 Northern 杂交检测各组肾皮质转化生长因子β1( T G Fβ1) m R N A 表达, Western 杂交检测各组肾皮质 T G Fβ1 及p21 C I P1 蛋白的表达。另外,血浆、肾皮、髓质血管紧张素转换酶( A C E) 活性由荧光分光光度法测定。结果 糖尿病大鼠一周后体重即明显下降( P< 0 .05) ,伴肾重/ 体重明显增加( P< 0 .05) ,血浆 A C E 活性有所下降而肾皮质 A C E 活性则有所上升。 Northern 杂交表明糖尿病组肾皮质 T G Fβ1m R N A 表达约比对照组上升63 .6 % 。肾皮质 T G Fβ1 及 P21 C I P1 蛋白表达也明显增加;苯那普利治疗一周对肾脏肥大有明显的抑制作用,对血浆、肾皮、髓质 A C E 活性抑制分别达89 .0 % 、70 .0 % 、70 .5 % ,对肾皮质 T G Fβ1 m R N A 及 T G Fβ1 和p21 C I P1 蛋白表达抑制分别达47 .7 % ,49 .5 % ,60
引用
收藏
页码:31 / 35
页数:5
相关论文
共 8 条
[1]  
The effect of A C Einhibitors on the expression of m atrix genes and the role p53 andp21 ( W A F1) in experimental renal fibrosis. Morrissey J J,Ishidoya S,Mccracken R,et al. Kidney International . 1996
[2]  
Angiotensin Ⅱstim ulated hypertrophy of L Lc P K1 cells depends on the induction of the cyclindependent kinase inhibitor p27 K I P L. W olf G,Stahl R A K. Kidney International . 1996
[3]  
Selective activation of theconverting enzy m e inhibition M K421 and co m parison of its activediacid form with captopril in different tissues of the rat. Unger T,Scholl B,Rascher W,et al. Biochem Phar macol . 1982
[4]  
Cyclin and cyclin depen dent kinase expression in the glo meruli of nor mal, diabetic and renalablatedrates (abstract). Shankland S J,Ham el P A,Scholey J W. J A m Soc Nephrol . 1994
[5]  
Glo merular expression ofp27 K I P1 in diabetic db/db m ouse : role of hyperglyce mia. Wolf G,Schroeder R,Thaiss F,et al. Kidney International . 1998
[6]  
Nifedipine versusfosinopril in uninephrecto mized diabetic rats. Anderson S,Rennke H G,Brenner B M,et al. Kidney International . 1992
[7]  
T G Fβ1induced cell cycle arrest in renal m esangial cells involves inibition ofcyclin Ecdk2 activation and retinoblasto m a protein phosphorylation. Schoecklmann H O,Rupprecht H D,Zauner I,et al. Kidney International . 1997
[8]  
Cellcycle control and renal disease. Shankland S J. Kidney International . 1997