苯那普利对糖尿病大鼠肾皮质p21CIP1蛋白表达的调节及其机制

被引：16

作者：

林善锬

吴永贵

余毅

周江华

机构：

[1] 上海医科大学华山医院肾内科!

来源：

中华内分泌代谢杂志 | 1999年 / 04期

关键词：

苯那普利; 糖尿病肾病; p21CIP1蛋白; 转化生长因子β;

D O I：

暂无

中图分类号：

R587.1 [糖尿病];

学科分类号：

摘要：

目的　研究苯那普利对糖尿病大鼠肾皮质ｐ２１ＣＩＰ１蛋白表达的调节及其机制。方法大鼠随机分单侧肾切除对照组、糖尿病组及糖尿病苯那普利（１０ｍｇ·ｋｇ－１ ·日－１，灌胃）治疗组。应用Ｎｏｒｔｈｅｒｎ杂交检测各组肾皮质转化生长因子β１（ＴＧＦβ１）ｍＲＮＡ表达，Ｗｅｓｔｅｒｎ杂交检测各组肾皮质ＴＧＦβ１及ｐ２１ＣＩＰ１蛋白的表达。另外，血浆、肾皮、髓质血管紧张素转换酶（ＡＣＥ）活性由荧光分光光度法测定。结果　糖尿病大鼠一周后体重即明显下降（Ｐ＜０．０５），伴肾重／体重明显增加（Ｐ＜０．０５），血浆ＡＣＥ活性有所下降而肾皮质ＡＣＥ活性则有所上升。Ｎｏｒｔｈｅｒｎ杂交表明糖尿病组肾皮质ＴＧＦβ１ｍＲＮＡ表达约比对照组上升６３．６％。肾皮质ＴＧＦβ１及Ｐ２１ＣＩＰ１蛋白表达也明显增加；苯那普利治疗一周对肾脏肥大有明显的抑制作用，对血浆、肾皮、髓质ＡＣＥ活性抑制分别达８９．０％、７０．０％、７０．５％，对肾皮质ＴＧＦβ１ｍＲＮＡ及ＴＧＦβ１和ｐ２１ＣＩＰ１蛋白表达抑制分别达４７．７％，４９．５％，６０

引用

页码：31 / 35

页数：5

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