人食管癌细胞株PTEN的激光共聚焦扫描显微镜检测

被引：1

作者：

杨海伟

朴仲贤

徐锡金

沈忠英

霍霞

机构：

[1] 汕头大学医学院中心实验室

[2] 汕头大学医学院中心实验室汕头

[3] 汕头

来源：

中国组织化学与细胞化学杂志 | 2004年 / 03期

关键词：

激光共聚焦扫描显微镜; PTEN; 食管癌;

D O I：

暂无

中图分类号：

R735.1 [食管肿瘤];

学科分类号：

100214 ;

摘要：

目的　对人胚食管上皮永生化细胞株SHEE、SHEEMT、食管癌细胞株EC8712中PTEN表达情况进行定量比较和定位观察。方法　采用激光共聚焦扫描显微镜光学切片和荧光探针的双重标记技术对三株细胞中PTEN的表达和分布情况进行检测。结果　人食管癌细胞中PTEN主要表达在细胞浆和细胞核 ,在人胚食管癌上皮永生化细胞株SHEE、SHEEMT主要表达在细胞浆 ,食管癌细胞EC8712中细胞核表达增多 ,差异有统计学意义 (P <0 0 1) ;PTEN在三种细胞株中表达强弱顺序为SHEE >SHEEMT >EC8712 ,差异有统计学意义 (P <0 0 1)。结论　PTEN在SHEE、SHEEMT和EC8712分化程度不同的细胞株中均表达 ,表达和分布位置与分化程度相关。

引用

页码：296 / 299

页数：4

共 9 条

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