未找到相关数据
紫外分光光度法下直接测定蛋白质溶液的浓度
被引：42
作者：
焦利敏
廖学品
石碧
机构：
[1] 四川大学皮革化学与工程教育部重点实验室
来源：
基金：
国家自然科学基金重点项目;
关键词：
分光光度法;
蛋白质;
测定;
A230法;
Lowry法;
D O I：
暂无
中图分类号：
O629.73 [蛋白质];
O657.32 [可见和紫外分光光度法];
学科分类号：
070302 [分析化学];
070303 [有机化学];
摘要：
The concentrations of bovine serum albumin,gliadin,bovine hemoglobim,mucin,lysozyme and proteins mixture in solutions were determined by spectrophotometric assay at 230 nm(A230 assay).For all the sample solutions of pure proteins,the linear range of A230assay was 25～250μg·mL-1,and salt had little influence on this method.For the solutions containing two kinds of proteins,the mass ratio of the proteins affected the result of determination.For the solutions of proteins mixture,the average recovery rate(n=6)of A230assay was 101.6%(99.04%～109.1%)with RSD=3.82%.As a result,a new effective method to determine protein concentration was suggested.
引用
收藏
页码：562 / 566
页数：5
相关论文