高压氧预适应对大鼠大脑中动脉闭塞后骨桥蛋白表达的影响

被引：10

作者：

黄宇星

胡胜利

邹永杰

陈图南

李强

李飞

冯华

机构：

[1] 第三军医大学西南医院神经外科,全军神经外科研究所,全军神经创伤防治重点实验室

来源：

第三军医大学学报 | 2013年 / 35卷 / 01期

关键词：

高压氧预适应; 大脑中动脉闭塞; 神经保护; 骨桥蛋白;

D O I：

10.16016/j.1000-5404.2013.01.006

中图分类号：

R362 [病理化学];

学科分类号：

100103 [病原生物学];

摘要：

目的观察高压氧预适应(hyperbaric oxygen preconditioning,HBOP)对大鼠大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)后的神经保护作用并初步探讨其对骨桥蛋白(osteopontin,OPN)表达的影响。方法雄性SD大鼠72只,分为假手术组,HBOP+假手术组,单纯MCAO组、HBOP+MCAO组,每组18只。采用大脑中动脉线栓法建立大鼠MCAO模型。于术后12 h取材,观察大鼠神经功能和脑梗死变化情况;HE染色光镜下观察缺血区脑组织的病理形态学改变。Western blot检测缺血区OPN的表达变化。结果神经功能缺损评分:术后12 h假手术组及HBOP+假手术组评分均为0分,单纯MCAO组(3.80±0.79)分,HBOP+MCAO组(2.50±0.97)分;平衡能力评分:术后12 h假手术组及HBOP+假手术组大鼠平衡能力评分均为5分,单纯MCAO组(1.50±1.08)分,HBOP+MCAO组(2.70±2.95)分;脑梗死率:术后12 h假手术组及HBOP+假手术组大鼠梗死率均为0,单纯MCAO组(49.22±7.07)%,HBOP+MCAO组(41.96±3.15)%。可见与单纯MCAO组比较,HBOP+MCAO组在术后12 h神经功能缺损评分明显降低(P<0.05),平衡能力评分显著提高(P<0.05);脑梗死率明显小于单纯MCAO组(P<0.05);HE染色见缺血区脑组织病理损伤明显减轻;Western blot检测提示OPN的表达显著增强。结论高压氧预适应可以诱导大鼠MCAO后的神经保护作用,其机制可能与HBOP引起的OPN表达增高有关。

引用

页码：15 / 19

页数：5

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