AnalysisoftheInhibitoryEffectofGypenosideonNa+,K+-ATPaseinRats'HeartandBrainandItsKinetics

被引：4

作者：

韩晓燕

卫洪波

张富程

机构：

[1] ResearchCenter,TheThirdAffiliatedHospital,SunYat-senUniversity

来源：

Chinese Journal of Integrative Medicine | 2007年 / 02期

关键词：

gypenoside; Na+; K+-ATPase; heart and brain microsome; enzyme kinetic;

D O I：

暂无

中图分类号：

R33 [人体生理学];

学科分类号：

1001 ;

摘要：

Objective:To study the effects of gypenoside(Gyp) on the activity of microsomal Na+,K+-ATPase in rat's heart and brain in vitro.Methods:The microsomal Na+,K+-ATPase was prepared from rat's heart and brain by differential centrifugation.The activity of microsomal Na+,K+-ATPase was assayed by colorimetric technique.Enzyme kinetic analysis method was used to analyze the effect of Gyp on the microsomal Na+,K+-ATPase of rats.Results:Gyp reversibly inhibited the brain and heart's microsomal Na+,K+-ATPase in a concentration-dependent manner,and showed a more potent effect on enzyme in the brain.The IC50 of Gyp for the heart and brain were 58.79±8.05 mg/L and 52.07± 6.25 mg/L,respectively.The inhibition was enhanced by lowering the Na+,or K+ concentrations or increasing the ATP concentration.Enzyme kinetic studies indicated that the inhibitory effect of Gyp on the enzyme is like that of competitive antagonist of Na +,the counter-competitive inhibitor for the substrate ATP,and the mixed-type inhibitor for K+.Conclusion:Gyp displays its cardiotonic and central inhibitory effects by way of inhibiting heart and brain's microsomal Na+,K+-ATPase activities in rats.

引用

页码：128 / 131

页数：4

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