神经病理性痛大鼠背根神经节P2X3受体表达及电生理学特性的改变

被引：33

作者：

刘晓红

曾俊伟

张金海

阮怀珍

机构：

[1] 第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所

来源：

第三军医大学学报 | 2008年 / 04期

关键词：

神经损伤; 背根神经节; P2X受体; 膜片钳; ATP;

D O I：

暂无

中图分类号：

R741.02 [神经病理学、病因学];

学科分类号：

100204 [神经病学];

摘要：

目的观察坐骨神经缩窄性损伤(CCI)致神经病理性痛后大鼠背根节神经元P2X3受体的表达和电生理学特性的变化。方法应用免疫组化技术观察CCI后不同时间相应节段(L4、L5、L6)脊髓及背根神经节P2X3受体表达的变化;全细胞膜片钳技术观察CCI后不同时间L4、L5、L6节段背根节神经元ATP反应电流的变化。结果CCI后7、14d,损伤同侧L4、L5、L6节段背根节P2X3免疫阳性神经元数量明显增加,而且染色加深,相应节段的脊髓背角浅层P2X3免疫反应性亦明显增加。CCI后7、14d,损伤同侧相应节段背根节产生ATP快反应电流的细胞数量明显增加,电流幅度亦增强。应用非选择性P2X受体拮抗剂Suramin能明显抑制ATP反应电流。结论坐骨神经缩窄性损伤致神经痛后同侧对应节段的背根节神经元P2X3受体表达明显增加,ATP快反应电流明显增强。

引用

页码：288 / 291

页数：4

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