流式细胞术

被引：16

作者：

谢小梅

许杨

机构：

[1] 江西中医学院

[2] 江西OAI联合研究院

来源：

中国生物工程杂志 | 2003年 / 09期

关键词：

流式细胞术;

D O I：

10.13523/j.cb.20030922

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

流式细胞术是一种综合应用光学、机械学、流体力学、电子计算机、细胞生物学、分子免疫学等学科技术 ,对高速流动的细胞或亚细胞进行快速定量测定和分析的方法。它一秒钟能分析几千个细胞 ,并同时测定细胞的多个参数 ,广泛应用于生物医学的许多领域 ,如测定细胞的特征(形态、膜电位等 )和细胞内pH ,细胞DNA、蛋白质含量、表面受体、Ca2 + 等。对生物工程学来说 ,了解细胞的这些参数尤为重要 ,因为它们能比用传统技术测得的数据更好地描述细胞群体。从流式细胞仪对细胞多种参数的测定及原理 ,到它在生物工程学中的应用等方面进行了介绍 ,并讨论了流式细胞术的局限性和面临的挑战。

引用

页码：100 / 104

页数：5

共 17 条

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