基于光谱自编码微球的多样品酶联免疫检测混合筛选

被引:3
作者
杜磊
刘烈雄
曹元成
机构
[1] 华中科技大学生命科学与技术学院
[2] 华中科技大学生命科学与技术学院 湖北武汉
关键词
光谱自编码; 苯乙烯; 微球; 酶联免疫检测; 高通量筛选;
D O I
10.13550/j.jxhg.2004.09.008
中图分类号
O657.3 [光化学分析法(光谱分析法)];
学科分类号
070302 ; 081704 ;
摘要
将苯乙烯及其同系物共聚合成具有不同特征光谱的光谱自编码微球,应用于酶联免疫检测(ELISA),AP酶标抗体筛选灵敏度达体积稀释比1∶160000。将20种编码微球与20个样品对映包被后混合,进行多样品ELISA混合筛选,分析阳性信号最强微球的光谱编码,得到了阳性反应样品的编号。成功开发了一种多样品的ELISA混合筛选技术。
引用
收藏
页码:667 / 670
页数:4
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