海洛因诱导大脑神经元凋亡的研究

被引：9

作者：

刘小山 ^{[1
]}

臧林泉 ^{[2
]}

蒿自睿 ^{[1
]}

李朝晖 ^{[1
]}

刘水平 ^{[1
]}

陈玉川 ^{[1
]}

吴敬东 ^{[3
]}

机构：

[1] 中山大学基础医学院法医系

[2] 广东药学院药科学院药理教研组

[3] 中山市公安局石歧分局

来源：

法医学杂志 | 2007年 / 01期

关键词：

海洛因; 大脑神经元; 凋亡;

D O I：

暂无

中图分类号：

D919 [法医学];

学科分类号：

0838 ; 1001 ; 1012 ;

摘要：

目的观察海洛因有无直接诱导培养大脑神经元凋亡的作用。方法神经元培养取自SD大鼠妊娠的胎鼠大脑皮质,培养7d后分别用不同浓度的海洛因(纯度80%)处理大脑神经元24h。用FDA法分析细胞存活率,Hoechst 33258荧光染色后观察凋亡的形态学改变,再用DNA琼脂糖凝胶电泳分析凋亡的生化特征。结果海洛因可剂量依赖性地降低神经元的存活率;荧光显微镜可见细胞核染为高亮蓝色的典型凋亡小体,其细胞核明显固缩、凝聚和断裂,且随海洛因剂量的增加,出现凋亡小体的细胞明显增多;不同浓度的海洛因处理大脑神经元,电泳图谱显示清晰的DNA梯带。结论海洛因可直接诱导大鼠大脑皮质神经元凋亡。

引用

页码：14 / 17

页数：4

共 14 条

[1]

Depolarization orglutamate receptor activation blocks apoptotic celldeath of culturd cerebellar granule neurons. Yang GM,Ni B,Weller M,et al. Brain Research Bulletin . 1994

[2]

Morphine alters the structure ofneurons in the nucleus accumbens and neocortex ofrats. Robinson TE,Kolb B. Synapse . 1999

[3]

Neurodegeneration in excitotoxicity,global cerebral is-chemia,and target deprivation:a perspective on the con-tributions of apoptosis and necrosis. Martin LJ,Al-Abdulla NA,Brambrink AM,et al. Brain Research Bulletin . 1998

[4]

Early brainchanges in HIV infection:neuropathological study of11 HIV seropositive,non-AIDS cases. Gray F,Lescs MC,Keohane C,et al. Journal of Neuropathology and Experimental Neurology . 1992

[5]

Toxiceffects of opioid and stimulant drugs on undifferenti-ated PC12 cells. Oliveira MT,Rego AC,Morgadinho MT,et al. Annals of the New York Academy of Sciences . 2002

[6]

Glucocorticoid-induced thymocyte apopto-sis is associated with endogenous endonucleaseactivation. Wyllic AH. Nature . 1980

[7]

Chronicmorphine induces visible changes in the morphology ofmesolimbic dopamine neurons. Sklair-Tavron L,Shi WX,Lane SB,et al. Proceedings of the National Academy of Sciences of the United States of America . 1996

[8]

Programmedcell death and the control of cell survival:lessonsfrom the nervous system. Raff MC,Barres BA,Burne JF,et al. Science . 1993

[9]

Apoptosis in the pathogenesis and treat-ment of disease. Thompson CB. Science . 1995

[10]

Morphine in-duces apoptosis of human microglia and neurons. Hu S,Sheng WS,Lokensgard JR.et al. Neuropharmacology . 2002

← 1 2 →