砀山酥梨叶片培养和植株再生

被引：16

作者：

赵竑博

徐凌飞

马锋旺

梁东

符轩畅

机构：

[1] 西北农林科技大学园艺学院

来源：

西北农业学报 | 2007年 / 02期

关键词：

梨; 叶片培养; 植株再生;

D O I：

暂无

中图分类号：

S661.2 [梨];

学科分类号：

090201 ;

摘要：

以砀山酥梨叶片为外植体,首次系统地研究了基本培养基、植物生长调节剂、碳源、暗培养时间和琼脂用量等对其再生不定芽的影响。结果表明,砀山酥梨叶片再生不定芽的适宜组合为NN69+6-BA 2.5 mg.L-1+IBA 0.3 mg.L-1+山梨醇20 g.L-1+琼脂8.0 g.L-1,暗培养20 d。叶片再生频率最高为83%,平均再生芽数2.17。叶片再生苗在生根培养基1/4 MS+IBA 0.5 mg.L-1+NAA0.3 mg.L-1+蔗糖20 g.L-1+琼脂6.0 g.L-1上已经生根。

引用

页码：153 / 157

页数：5

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