PCR-DGGE技术分析蛋鸡MHC基因对肠道细菌种群结构的影响

被引：13

作者：

倪学勤 ^{[1
]}

Joshua Gong ^{[2
]}

Hai Yu ^{[2
]}

Shayan Sharif ^{[3
]}

曾东 ^{[1
]}

机构：

[1] 四川农业大学动物医学院

[2] Food Research Program,Agriculture and Agri-Food Canada

[3] Department of Animal and Poultry Science,University of Guelph

来源：

中国农业科学 | 2009年 / 42卷 / 07期

关键词：

肠道菌群; MHC; 变性梯度凝胶电泳; 蛋鸡;

D O I：

暂无

中图分类号：

S831 [鸡];

学科分类号：

0905 ;

摘要：

【目的】研究蛋鸡MHC(Major Histocompatibility Complex,主要组织相容性复合物)基因对不同周龄和不同部位肠道细菌种群结构的影响。【方法】使用基于16S rDNA的PCR-DGGE技术,结合特异性和共性条带割胶回收DNA进行克隆和测序,对2,4,6和8周龄商品蛋鸡和15I5系蛋鸡嗉囊、十二指肠、空肠、回肠和盲肠内含物细菌群落的结构和多样性进行比较,鉴定8周龄蛋鸡部分特异性和共性群落成员。【结果】商品蛋鸡和15I5系蛋鸡盲肠细菌的多样性最高,其次是回肠和空肠,嗉囊和十二指肠的细菌多样性比较低,且随着蛋鸡周龄增加,各肠段细菌多样性呈上升趋势,MHC基因对细菌多样性无显著影响。DGGE图谱聚类分析表明,蛋鸡MHC基因对4、6周龄肠道细菌的影响最大,对2周龄影响最小;而且MHC基因对消化道前段细菌的影响小于对后段影响。序列分析发现,8周龄商品蛋鸡和15I5系蛋鸡均有大量的三得利乳杆菌(Lactobacillus suntoryeus),是蛋鸡消化道前段的优势细菌;商品蛋鸡空肠、回肠和盲肠中的特异性条带主要是Ochrobactrum spp,索氏梭菌(Clostridium sordellii),超巨巨单胞菌(Megamonas hypermegale)和大量不可培养的细菌;15I5系蛋鸡的特异性条带主要是不可培养的细菌。【结论】蛋鸡MHC基因可影响不同周龄、不同肠段细菌群落的结构。

引用

页码：2564 / 2571

页数：8

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