敲低SOX5对骨关节炎软骨细胞生物学功能的影响

被引：7

作者：

张磊

宁玉辉

李国顺

何涛

牟宗友

李明

郭金泉

机构：

[1] 德州人民医院关节外科

来源：

中国骨与关节杂志 | 2017年 / 6卷 / 12期

关键词：

骨关节炎; 软骨细胞; SOX5; MMPs; 细胞凋亡;

D O I：

暂无

中图分类号：

R684.3 [关节炎];

学科分类号：

100220 [骨科学];

摘要：

目的探讨转录因子SOX5在骨关节炎软骨细胞中的生物学功能。方法分离培养人骨关节炎(osteoarthritis,OA)软骨细胞;运用脂质体2000转染法将si SOX5转染OA软骨细胞(OA-si SOX5),以转染NCsi RNA为阴性对照(OA-NCsi RNA);运用Real time PCR检测转染的OA软骨细胞中SOX5、白介素6(IL-6)、白介素1β(IL-1β)、II型胶原(COL2A1)及蛋白多糖(ACAN)m RNA的表达水平;酶联免疫法(ELISA)检测OA软骨细胞培养上清中IL-6和IL-1β浓度;Western blot检测SOX5、基质金属蛋白酶1(MMP-1)和MMP-13蛋白表达水平;运用Annexin V-FITC及流式细胞术检测细胞凋亡。结果 OA-si SOX5细胞中IL-6(0.72±0.05)和IL-1βm RNA(0.64±0.07)表达显著低于OA-NCsi RNA细胞(1.32±0.08、1.64±0.09)(P<0.05);COL2A1(1.27±0.08)和ACAN m RNA(2.38±0.17)显著高于OA-NCsi RNA细胞(0.58±0.04,1.25±0.10),(P<0.05);OA-si SOX5细胞中IL-6(175.2±14.5)pg/ml和IL-1β(102.6±20.3)pg/ml浓度显著低于OA-NCsi RNA细胞[(584.6±74.5)pg/ml和(268.4±25.3)pg/ml)](P<0.05);细胞凋亡率OA-si SOX5[(3.04±0.86)%]显著低于OA-NCsi RNA细胞[(18.35±2.74)%](P<0.05);OA-si SOX5细胞中MMP-1(0.53±0.05)和MMP-13蛋白水平(0.46±0.08)明显低于OA-NCsi RNA细胞(1.95±0.11、2.48±0.24)(P<0.05)。结论下调SOX5表达可能通过抑制基质金属蛋白酶的表达、促进细胞外基质的合成与分泌、抑制细胞凋亡和炎症反应进而缓减OA的发展。

引用

页码：932 / 937

页数：6

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