抗TNF-α和IL-1βIgY抗体治疗豚鼠过敏性鼻炎机理研究

被引：13

作者：

朱喜玲 ^{[1
]}

胡微煦 ^{[2
]}

吴梨华 ^{[3
]}

文珠 ^{[4
]}

何丹 ^{[3
]}

吴晓牧 ^{[3
]}

胡国柱 ^{[3
]}

机构：

[1] 南昌大学医学院研究院

[2] 复旦大学附属中山医院放疗科

[3] 江西省人民医院临床医学研究所

[4] 江西省医学科学研究院血液研究室

来源：

中国免疫学杂志 | 2014年 / 30卷 / 10期

关键词：

过敏性鼻炎; 豚鼠; 卵白蛋白; 肿瘤坏死因子; 白细胞介素;

D O I：

暂无

中图分类号：

R765.21 [鼻炎];

学科分类号：

100213 [耳鼻咽喉科学];

摘要：

目的:探讨抗TNF-α和IL-1βIgY治疗豚鼠过敏性鼻炎的作用及机制。方法:随机分正常对照组(C组,17只)、模型组(M组,27只)、0.1%抗TNF-α和IL-1βIgY治疗组(Z1组,21只)、丙酸氟替卡松治疗组(Z2组,21只);应用卵清白蛋白建立豚鼠过敏性鼻炎模型;分别在治疗后2、4、8 h进行鼻灌洗和支气管肺灌洗,收集鼻灌洗液(NLF)和支气管肺泡灌洗液(BALF),观察炎症细胞;HE染色观察鼻黏膜和肺组织病理改变;免疫组化分析其炎症因子原位表达情况。结果:M组鼻黏膜可见大量嗜酸性粒细胞浸润和炎症改变,肺间质水肿、肺间隔和支气管平滑肌增厚,嗜酸性粒细胞浸润,Z1组和Z2组炎症病理改变明显减轻。NLF和BALF中嗜酸性粒细胞、淋巴细胞、中性粒细胞在Z1和Z2组显著少于M组(P<0.05)。Z1组鼻黏膜IL-1β和肺组织IL-1β和TNF-α从2 h开始,而IL-5和IL-33从4 h开始表达显著低于M组(P<0.05)。结论:抗TNF-α和IL-1βIgY滴鼻治疗显著减轻了豚鼠过敏性鼻炎伴过敏性支气管哮喘的炎症病理反应,抑制了嗜酸性粒细胞浸润及炎症细胞因子表达。

引用

页码：1353 / 1359

页数：7

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