单克隆抗体纯化研究进展

被引：18

作者：

应国清

祝骥

易喻

梅建凤

陈建澍

机构：

[1] 浙江工业大学药学院

来源：

中国医药工业杂志 | 2008年 / 10期

关键词：

单克隆抗体; 纯化; 综述;

D O I：

暂无

中图分类号：

R392 [医学免疫学];

学科分类号：

100108 [医学免疫学];

摘要：

综述了单克隆抗体的纯化技术,讨论了各单元操作的关键问题和解决方法,并重点介绍了模拟移动床色谱、双水相萃取、膜色谱等操作简单、能连续生产、低成本的纯化技术。

引用

页码：777 / 782

页数：6

共 15 条

[1]

Affinity purification of IgG monoclonal antibodies using the D-PAM synthetic ligand: chromatographic comparison with protein A and thermodynamic investigation of the D-PAM/IgG interaction.[J].Biagio D'Agostino;Piero Bellofiore;Tiziana De Martino;Carmine Punzo;Vincenzo Rivieccio;Antonio Verdoliva.Journal of Immunological Methods.2008, 1

[2]

Accelerated purification process development of monoclonal antibodies for shortening time to clinic.[J].Takashi Ishihara;Toshihiko Kadoya.Journal of Chromatography A.2007, 1

[3]

Step gradients in 3-zone simulated moving bed chromatography.[J].Lars Christian Keβler;Ludmila Gueorguieva;Ursula Rinas;Andreas Seidel-Morgenstern.Journal of Chromatography A.2007, 1

[4]

pH–conductivity hybrid gradient cation-exchange chromatography for process-scale monoclonal antibody purification.[J].Joe X. Zhou;Shinta Dermawan;Felix Solamo;Greg Flynn;Rosalind Stenson;Tim Tressel;Sam Guhan.Journal of Chromatography A.2007, 1

[5]

Protein aggregation kinetics during Protein A chromatography.[J].Abhinav A. Shukla;Priyanka Gupta;Xuejun Han.Journal of Chromatography A.2007, 1

[6]

Cored anion-exchange chromatography media for antibody flow-through purification [J].

Wang, Chen ;

Soice, Neil P. ;

Ramaswamy, Senthilkumar ;

Gagnon, Brian A. ;

Umana, Joaquin ;

Cotoni, Kristen A. ;

Bian, Nanying ;

Cheng, Kwok-Shun C. .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1155 (01) :74-84

[7]

Optimisation of aqueous two-phase extraction of human antibodies [J].

Azevedo, Ana M. ;

Rosa, Paula A. J. ;

Ferreira, I. Filipa ;

Aires-Barros, M. Raquel .

JOURNAL OF BIOTECHNOLOGY, 2007, 132 (02) :209-217

[8]

Purification of humanized monoclonal antibodies by membrane-based hybrid bioseparation technique.[J].Lu Wang;Dharmesh M. Kanani;Raja Ghosh.Journal of Immunological Methods.2006, 1

[9]

Discontinuous and continuous separation of the monomeric and dimeric forms of human bone morphogenetic protein-2 from renaturation batches [J].

Gueorguieva, Ludmila ;

Vallejo, Luis Felipe ;

Rinas, Ursula ;

Seidel-Morgenstern, Andreas .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1135 (02) :142-150

[10]

New Q membrane scale-down model for process-scale antibody purification.[J].Joe X. Zhou;Tim Tressel;Uwe Gottschalk;Felix Solamo;Andre Pastor;Shinta Dermawan;Tony Hong;Oscar Reif;Jeff Mora;Fred Hutchison;Michael Murphy.Journal of Chromatography A.2006, 1

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