腹腔注射干扰素γ对哮喘小鼠肺组织GATA-3及气道炎症的影响

被引：11

作者：

黄艳

陈吉泉

修清玉

机构：

[1] 第二军医大学长征医院呼吸内科

来源：

第二军医大学学报 | 2005年 / 10期

关键词：

干扰素γ; 哮喘; GATA-3; 注射,腹腔内;

D O I：

10.16781/j.0258-879x.2005.10.018

中图分类号：

R562.25 [];

学科分类号：

摘要：

目的:研究腹腔注射IFNγ对支气管哮喘的防治作用及其机制。方法:BALB/c小鼠随机分为三组,正常对照组(A组,12只)、哮喘模型组(B组,12只)、腹腔注射IFNγ组(C组,12只),B组、C组采用卵蛋白(OVA)和氢氧化铝建立哮喘模型,分别于第23天至第30天每次激发前腹腔注射0.25mlPBS液和IFNγ5μg,1次/d。第31天取肺泡灌洗液(BALF)测定细胞成分、IL4、IL5浓度,取血测定血浆IgE水平,观察肺组织病理学变化及GATA3的表达。结果:(1)C组BALF中的嗜酸粒细胞(EOS)(0.3±0.2)明显低于B组(21.1±6.7),P<0.01;(2)C组BALF中的IL4(3.0±2.5)和IL5(11.4±3.2)的水平明显低于B组的IL4(90.5±22.4)和IL5(55.6±10.7),P<0.01;(3)C组血浆中总IgE(24.3±7.6)和OVA特异性IgE(12.3±3.6)水平显著低于B组的IgE(67.2±9.7)和OVA特异性IgE(34.6±7.8),P<0.01;(4)HE染色示B组支气管平滑肌肥厚,黏膜充血、水肿,黏膜层增厚,并有EOS为主的炎性细胞浸润,管腔内可见黏液栓,支气管壁周围有EOS为主的炎性细胞浸润,而C组小鼠的上述炎性改变则明显减轻;(5)免疫组化显示B组肺组织中GATA3的表达明显增加,而C组GATA3的表达明显减少。结论:腹腔注射IFNγ可以抑制哮喘鼠IL4、IL5的合成、抑制EOS在气道内的炎性浸润及血浆中总IgE和OVA特异性的IgE水平,其机制可能与IFNγ阻断GATA3的表达,从而继发抑制Th2型反应有关。

引用

页码：1152 / 1156

页数：5

共 11 条

[1]

Chen XM,Wang CZ,Li SP,et al.Establishment of sensitized asthma model in mice in and CD4+ T lymphocyte isolation from spleens. Acta Academiae Med Militaris Tertiae . 2001

[2]

IwamotoI,NakajimaH,EndoH ,etal.Interferongammaregulatesantigen inducedeosinophilrecruitmentintothemouseairwaysbyinhibitingtheinfiltrationofCD4+Tcells. The Journal of Experimental Medicine . 1993

[3]

Ray A,Cohn L.Th2 cells and GATA-3 in asthma: new insights into the regulation of airway inflammation. The Journal of Clinical Investigation . 1999

[4]

Kips JC,Brusselle GJ,Joos GF,et al.Interleukin-12 inhibits antigen-induced airway hyper responsiveness in mice. American Journal of Respiratory and Critical Care Medicine . 1996

[5]

Yoshida M,Leigh R,Matsumoto K,et al.Effect of interferongamma on allergic airway responses in interferon-gamma-deficient mice. American Journal of Respiratory and Critical Care Medicine . 2002

[6]

Tamauchi H,Terashima M,Ito M,et al.Evidence of GATA-3dependent Th2 commitment during the in vivo immune response. International Immunology . 2004

[7]

DickensheetsHL,DonnellyRP.InhibitionofIL4inducible geneexpressioninhumanmonocytesbytypeⅠandtypeⅡin terferon. Journal of Leukocyte Biology . 1999

[8]

Spapenko A,Leipe J,Niesner V,et al.GATA-3 in human T cell helper type 2 development. The Journal of Experimental Medicine . 2004

[9]

Barneses PJ,Lim S.Inhibitory cytokine in asthma. Mol Med Toda . 1998

[10]

HofstraCL,ArkIV,HofmanG,etal.Differentialeffectsofen dogenousandexogenousinterferonγonimmunoglobulinE,cellularinfiltration,andairwayresponsivenessinamurinemodelofallergicasthma. AmJCellMolBiol . 1998

← 1 2 →