葡萄糖和木糖双底物生物转化生产2,3-丁二醇和氢气的代谢计量分析

被引：7

作者：

马成伟

孙亚琴

修志龙

机构：

[1] 大连理工大学环境与生命学院

来源：

生物加工过程 | 2006年 / 03期

关键词：

2,3-丁二醇; 氢; 葡萄糖; 木糖; 代谢计量分析;

D O I：

暂无

中图分类号：

TQ223.162 []; TQ116.2 [氢气];

学科分类号：

0817 ;

摘要：

以Klebsiella pneumoniae利用葡萄糖和木糖双底物生物转化生产2,3-丁二醇和氢气过程为研究对象,对其进行代谢计量分析。分析结果显示:2,3-丁二醇和氢气相对于底物葡萄糖和木糖的质量收率依赖于还原能力NADH2氧化磷酸化的分率(δ)。当δ=27时,即在总还原能力NADH2中有27mol NADH2被氧化磷酸化,剩余部分用来产生氢气,呼吸商为14时,2,3-丁二醇和氢气的最优质量收率分别为50%和0.8%;而当δ=1,即还原能力NADH2全部被氧化磷酸化、不产生氢气,呼吸商为4时,2,3-丁二醇的质量收率为37.5%;2,3-丁二醇和氢气的质量收率与底物中葡萄糖和木糖的比值无关。而氢气的摩尔收率与底物中葡萄糖和木糖的比值相关,当底物全部是葡萄糖或木糖时,其最优摩尔收率分别为71%和60%。该分析结果为葡萄糖和木糖双底物生物转化生产2,3-丁二醇过程的实验研究奠定了理论基础。

引用

页码：44 / 50

页数：7

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