水稻ABCB转运蛋白基因的分子进化和表达分析

被引：39

作者：

徐杏

邱杰

徐扬

徐辰武

机构：

[1] 扬州大学作物遗传生理省级重点实验室/教育部植物功能基因组学重点实验室

来源：

中国水稻科学 | 2012年 / 26卷 / 02期

关键词：

水稻; ABC转运蛋白; 进化; 表达分析;

D O I：

暂无

中图分类号：

S511 [稻];

学科分类号：

090104 [作物信息科学与技术];

摘要：

系统鉴定了水稻和拟南芥中27个和29个ABC家族B亚族(ABCB)基因,发现其外显子数目、编码蛋白质的长度和分子量在两种植物中均存在较大变异,而等电点分布的变化较小。系统发育分析把该亚族蛋白分为4个亚组,说明其功能可能已经发生了分化;在水稻和拟南芥中各鉴定了6对和9对旁系同源基因,说明单、双子叶植物分离之后,该亚族在水稻和拟南芥中均以物种特异的方式进行了扩张。片段复制和串联重复是水稻ABC家族扩张的主要机制。多序列比对显示,核苷酸结合域(NBD)的Walker A、Walker B和ABC域基序均高度保守,而Q环和H环的保守性略差;跨膜结构域(TMD)在不同蛋白之间和同一蛋白内没有明显的保守性。表达模式分析表明,水稻ABCB基因的表达具有明显的组织特异性,不同基因的表达特征已经发生分化。非生物胁迫下的表达分析显示多数水稻ABCB基因受到至少一种非生物胁迫因素的调控。旁系同源基因Ka/Ks分析表明,净化选择在水稻ABCB基因复制后功能的保留中发挥了重要作用。

引用

页码：127 / 136

页数：10

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