白术提取物苍术酮对脂多糖诱导的BV2细胞神经炎性影响及相关机制研究

被引：57

作者：

李静 ^{[1
,2
]}

金倫喆 ^{[2
]}

金洪光 ^{[3
]}

机构：

[1] 九江学院附属医院药剂科

[2] 韩国圆光大学药学院

[3] 九江学院

来源：

天然产物研究与开发 | 2020年 / 32卷 / 05期

关键词：

苍术酮; 脂多糖; BV2细胞; 神经炎症;

D O I：

10.16333/j.1001-6880.2020.5.015

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

研究旨在白术提取物苍术酮（AT）对脂多糖（LPS）诱导的BV2细胞抗炎作用,并探讨其相关机制。提取苍术酮并进行结构鉴定;以LPS诱导BV2细胞建立炎症模型;四甲基偶氮唑蓝（MTT）法检测细胞存活率;Griess反应检测细胞上清液中NO水平;ELISA法检测细胞上清中PGE2、IL-6、TNF-α水平;Western Blot法检测COX-2、iNOS、MAPK、NF-κB蛋白表达。结果表明LPS诱导BV2细胞后NO、PGE2、IL-6、TNF-α水平和COX-2及iNOS蛋白表达显著增高,苍术酮预处理后均显著降低。进一步研究表明苍术酮可显著降低LPS诱导BV2细胞ERK、JNK、NF-κB蛋白表达。说明苍术酮能够有效预防LPS诱导BV2细胞神经炎性反应,机制与抑制炎症通路相关。

引用

页码：826 / 830+758 +758

页数：6

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